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human embryonic stem cell line h1  (WiCell Research Institute Inc)


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    WiCell Research Institute Inc human embryonic stem cell line h1
    Human Embryonic Stem Cell Line H1, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 99/100, based on 962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wa01+h1+human+embryonic+stem+cell+line/WA01/pmc12901785-36-7-15
    Average 99 stars, based on 962 article reviews
    human embryonic stem cell line h1 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title: TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD
    Article Snippet: Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).. The homozygous KCNQ2 ∆E5 engineered cell line was created using the WA01 (H1) human embryonic stem cell line from WiCell.. The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.

    Article Title: Autophagy modulates cell fate decisions during lineage commitment
    Article Snippet: Models: Cell Lines , , . .. WA01 (H1) Human Embryonic Stem Cell Line (NIH Approval Number: NIHhESC-10-0043) , WiCell , N/A. .. hiPSC Line AG27 derived from fribrobla

    Derivative Assay:

    Article Title: TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD
    Article Snippet: Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).. The homozygous KCNQ2 ∆E5 engineered cell line was created using the WA01 (H1) human embryonic stem cell line from WiCell.. The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.

    Article Title: Autophagy modulates cell fate decisions during lineage commitment
    Article Snippet: Models: Cell Lines , , . .. WA01 (H1) Human Embryonic Stem Cell Line (NIH Approval Number: NIHhESC-10-0043) , WiCell , N/A. .. hiPSC Line AG27 derived from fribrobla

    Knock-Out:

    Article Title: TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD
    Article Snippet: Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).. The homozygous KCNQ2 ∆E5 engineered cell line was created using the WA01 (H1) human embryonic stem cell line from WiCell.. The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.

    Article Title: Autophagy modulates cell fate decisions during lineage commitment
    Article Snippet: Models: Cell Lines , , . .. WA01 (H1) Human Embryonic Stem Cell Line (NIH Approval Number: NIHhESC-10-0043) , WiCell , N/A. .. hiPSC Line AG27 derived from fribrobla

    CRISPR:

    Article Title: TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD
    Article Snippet: Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).. The homozygous KCNQ2 ∆E5 engineered cell line was created using the WA01 (H1) human embryonic stem cell line from WiCell.. The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.

    Article Title: Autophagy modulates cell fate decisions during lineage commitment
    Article Snippet: Models: Cell Lines , , . .. WA01 (H1) Human Embryonic Stem Cell Line (NIH Approval Number: NIHhESC-10-0043) , WiCell , N/A. .. hiPSC Line AG27 derived from fribrobla

    Bicinchoninic Acid Protein Assay:

    Article Title: TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD
    Article Snippet: Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).. The homozygous KCNQ2 ∆E5 engineered cell line was created using the WA01 (H1) human embryonic stem cell line from WiCell.. The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.

    Article Title: Autophagy modulates cell fate decisions during lineage commitment
    Article Snippet: Models: Cell Lines , , . .. WA01 (H1) Human Embryonic Stem Cell Line (NIH Approval Number: NIHhESC-10-0043) , WiCell , N/A. .. hiPSC Line AG27 derived from fribrobla

    Protease Inhibitor:

    Article Title: TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD
    Article Snippet: Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).. The homozygous KCNQ2 ∆E5 engineered cell line was created using the WA01 (H1) human embryonic stem cell line from WiCell.. The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.

    Article Title: Autophagy modulates cell fate decisions during lineage commitment
    Article Snippet: Models: Cell Lines , , . .. WA01 (H1) Human Embryonic Stem Cell Line (NIH Approval Number: NIHhESC-10-0043) , WiCell , N/A. .. hiPSC Line AG27 derived from fribrobla

    SYBR Green Assay:

    Article Title: TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD
    Article Snippet: Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).. The homozygous KCNQ2 ∆E5 engineered cell line was created using the WA01 (H1) human embryonic stem cell line from WiCell.. The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.

    Article Title: Autophagy modulates cell fate decisions during lineage commitment
    Article Snippet: Models: Cell Lines , , . .. WA01 (H1) Human Embryonic Stem Cell Line (NIH Approval Number: NIHhESC-10-0043) , WiCell , N/A. .. hiPSC Line AG27 derived from fribrobla

    Expressing:

    Article Title: TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD
    Article Snippet: Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).. The homozygous KCNQ2 ∆E5 engineered cell line was created using the WA01 (H1) human embryonic stem cell line from WiCell.. The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.

    Article Title: Autophagy modulates cell fate decisions during lineage commitment
    Article Snippet: Models: Cell Lines , , . .. WA01 (H1) Human Embryonic Stem Cell Line (NIH Approval Number: NIHhESC-10-0043) , WiCell , N/A. .. hiPSC Line AG27 derived from fribrobla

    Plasmid Preparation:

    Article Title: TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD
    Article Snippet: Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).. The homozygous KCNQ2 ∆E5 engineered cell line was created using the WA01 (H1) human embryonic stem cell line from WiCell.. The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.

    Article Title: Autophagy modulates cell fate decisions during lineage commitment
    Article Snippet: Models: Cell Lines , , . .. WA01 (H1) Human Embryonic Stem Cell Line (NIH Approval Number: NIHhESC-10-0043) , WiCell , N/A. .. hiPSC Line AG27 derived from fribrobla

    Software:

    Article Title: TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD
    Article Snippet: Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).Stem cells at Northwestern University were maintained on plates coated with Matrigel Basement Membrane Matrix (Corning, CLS354234) in mTeSR or mTeSR Plus media (StemCell Technologies, 100-0276) and passaged with Accutase (Gibco, A11105).. The homozygous KCNQ2 ∆E5 engineered cell line was created using the WA01 (H1) human embryonic stem cell line from WiCell.. The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.The iPS cell lines 18a and KOLF2.1J were used to differentiate spinal MNs and cortical NGN2-based neurons at Northwestern University.

    Article Title: Autophagy modulates cell fate decisions during lineage commitment
    Article Snippet: Models: Cell Lines , , . .. WA01 (H1) Human Embryonic Stem Cell Line (NIH Approval Number: NIHhESC-10-0043) , WiCell , N/A. .. hiPSC Line AG27 derived from fribrobla



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    a –c For each species: circular projection of CellRank-calculated fate probabilities for each cell toward the terminal states (outer labels); and UMAPs detailing endocrine progenitors branching, with the integrated pancreas atlas showing NEUROG3 expression (red) and endocrine progenitor cluster boundaries (black), and an insert showing endocrine progenitor subclusters with overlaid CellRank-inferred trajectories (arrows). d Line plots showing the cumulative number of CellRank-derived beta-cell (top) and alpha-cell (bottom) lineage drivers in mouse and pig that overlap with human orthologs, plotted across correlation score thresholds (Supplementary Data ). Solid lines show significant driver numbers (Benjamini-Hochberg FDR-corrected p-value < 0.05). Shaded regions indicate the number of genes obtained when using the lower and upper bounds of the 95% confidence interval for the corresponding correlation score. e, f Heatmaps displaying modeled gene expression patterns for human beta-cell ( e ) and alpha-cell ( f ) lineage driver gene clusters (identified by hierarchical clustering) across pig, human, and mouse along pseudotemporal trajectories (left to right: 0 → 1). Annotations indicate species-conserved pathways and representative genes for each cluster. (corr., correlation; n, number of conserved genes that are expressed in > 20% of endocrine progenitor subclusters) g –i Comparison of NEUROG3 TF targets identified in human/pig pancreas and hESC model (conserved targets in blue). g, h Circular GRN graphs showing first- and second-order NEUROG3 targets from human scGLUE-jointed scRNA/ATAC-seq data ( g ) and pig multiomic data ( h ). Nodes represent TFs. Edge color indicates regulatory interaction types (orange, activating; gray, inhibiting); i NEUROG3 TF targets in hESC model with inducible NEUROG3 expression. ChIP-seq-identified direct targets are shaded. Differentially expressed TFs comparing cells with/without NEUROG3 expression are indicated by arrows (red, upregulated; blue, downregulated). a – f scRNA-seq of pancreatic cells from wild-type and INS -eGFP pigs. h Multiome analysis of pancreatic cells from PTF1A -codon-improved-Cre/ROSA-mTmG pigs. Detailed sample information is provided in Supplementary Data .

    Journal: Nature Communications

    Article Title: A multimodal cross-species comparison of pancreas development

    doi: 10.1038/s41467-025-64774-4

    Figure Lengend Snippet: a –c For each species: circular projection of CellRank-calculated fate probabilities for each cell toward the terminal states (outer labels); and UMAPs detailing endocrine progenitors branching, with the integrated pancreas atlas showing NEUROG3 expression (red) and endocrine progenitor cluster boundaries (black), and an insert showing endocrine progenitor subclusters with overlaid CellRank-inferred trajectories (arrows). d Line plots showing the cumulative number of CellRank-derived beta-cell (top) and alpha-cell (bottom) lineage drivers in mouse and pig that overlap with human orthologs, plotted across correlation score thresholds (Supplementary Data ). Solid lines show significant driver numbers (Benjamini-Hochberg FDR-corrected p-value < 0.05). Shaded regions indicate the number of genes obtained when using the lower and upper bounds of the 95% confidence interval for the corresponding correlation score. e, f Heatmaps displaying modeled gene expression patterns for human beta-cell ( e ) and alpha-cell ( f ) lineage driver gene clusters (identified by hierarchical clustering) across pig, human, and mouse along pseudotemporal trajectories (left to right: 0 → 1). Annotations indicate species-conserved pathways and representative genes for each cluster. (corr., correlation; n, number of conserved genes that are expressed in > 20% of endocrine progenitor subclusters) g –i Comparison of NEUROG3 TF targets identified in human/pig pancreas and hESC model (conserved targets in blue). g, h Circular GRN graphs showing first- and second-order NEUROG3 targets from human scGLUE-jointed scRNA/ATAC-seq data ( g ) and pig multiomic data ( h ). Nodes represent TFs. Edge color indicates regulatory interaction types (orange, activating; gray, inhibiting); i NEUROG3 TF targets in hESC model with inducible NEUROG3 expression. ChIP-seq-identified direct targets are shaded. Differentially expressed TFs comparing cells with/without NEUROG3 expression are indicated by arrows (red, upregulated; blue, downregulated). a – f scRNA-seq of pancreatic cells from wild-type and INS -eGFP pigs. h Multiome analysis of pancreatic cells from PTF1A -codon-improved-Cre/ROSA-mTmG pigs. Detailed sample information is provided in Supplementary Data .

    Article Snippet: The parent human embryonic stem cell (hESC) line WA01 (H1) was obtained from WiCell.

    Techniques: Expressing, Derivative Assay, Gene Expression, Comparison, ChIP-sequencing